Daya Hidup Spermatozoa Sapi Limousin yang Dipreservasi dengan Metode Water Jacket dan Free Water Jacket (SPERMATOZOA VIABILITY OF LIMOUSIN CATTLE PRESERVED WITH WATER JACKET AND FREE WATER JACKET METHOD)

Abstract

This study aimed to determine the effect of different methods of saving the storage by using a waterjacket and a free water jacket to quality semen Limousin cattle after being stored at a temperature of 5°C.Semen used the criteria motility e” + + collected two times a week. Semen evaluated macroscopically andmicroscopically and diluted in the ratio 1: 1 using the Cauda Epididymal Plasma (CEP-2) diluent added10% egg yolk. Stored in the refrigerator temperature 5°C and semen quality  was observed immediatelyafter dilution, 1 hour, 2 hours, 3 hours, 24 hours, 48 hours and 72 hours. The results showed that themethod of free storage waterjacket better in maintaining the percentage of motility, viability, abnormalityand plasma membrane intact spermatozoa